1.Stitching changes: beside the AREF sutural added two I2C pins (SDA and SCL, only Analog copy of 4 and 5, are not additional I2C interface), in addition, beside the RESET also added two pins, one is IOREF, can let the extension board to board, load voltage, the stitching is tell extension board currently on-board voltage is how many (such as UNO is 5 v, can is regarded as a copy of the power pins, does not provide a level), the other one is after treated with placeholder pins.
2.The RESET circuit is more stable. RESET button position is changed, and moved to near the USB interface of voids, it is more convenient.
3.ATmega16U2 instead of 8 u2, this does not mean that there are 16 k flash R3 can make your code run faster, the update service for the USB interface chip, it make UNO can simulate USB HID in theory, such as MIDI / Joystick / Keyboard.